Flow Cytometry

What is Flow Cytometry?

Flow cytometry uses fluorescent probes to identify and characterize cells or particles in suspension (e.g.听cells, nuclei or chromosomes) by virtue of size, granularity and fluorescence (auto-fluorescence or fluorescence after immunolabelling or staining). Cells or particles tagged with fluorescent molecules enter the flow cytometer via a fluid stream. The cells then pass by a laser, which emits a specific wavelength of light. The fluorescent probes are excited by the laser and then emit light. The fluorescent signal is detected and amplified, then translated into an electronic signal, which is sent to the computer. The result is a visual presentation describing an individual or group of cellular events. The cells or particles can be separated by sorting, or the information can be collected and analyzed.

BD FACSAria Cell Sorter

罢丑别听BD FACSAria Cell Sorter听is located in Higgins 458.听Operator only available Monday揊riday, 10 AM4 PM.

 Sony SH800


罢丑别听Sony SH800, located in Higgins 458, is a high-speed, benchtop, cell sorter equipped with 2-lasers capable of 8 parameters (6 fluorescence channels, and forward and side scatter). It can sort up to 2 populations simultaneously, as well as plate sorting.

Advances in cell sorting instrumentation have enabled this technology to be applied to a wider array of cell biology areas, such as hematology, immunology, microbiology, cellular signaling, and parasitology.


Facility Documentation

Flow Cytometry Biosafety Protocols

Cell Sorting Request Form

S3e听Cell Sorter Quick Guide

Please note that all work performed in 51动漫 core facilities and recharge centers should always be appropriately acknowledged.听 If you are publishing or presenting data acquired in 51动漫 core facilities and recharge centers, please include the following statement in the Acknowledgement section of your manuscript/poster/presentation, "The authors would like to thank the Boston College <insert facility name> for assistance with the work presented in this paper/poster/presentation*."
* Delete as appropriate

Patrick Autissier

Patrick Autissier, Ph.D.
Director, Flow Cytometry Facility

I am a biologist with over 25 years of flow cytometry and cell sorting experience. I received my Ph.D. from the C.N.A.M. in Paris, France, where I developed a 12-color flow cytometry panels to study immune cells in both humans and monkeys. I authored or co-authored more than 40 scientific articles.

My family and I moved to the US in 2001, where I was hired by the Division of Viral Pathogenesis at Beth Israel Deaconess Medical Center in Boston, to run the Cell Sorting Facility. I worked there for 7 years prior to joining Boston College where I am in charge of the Flow Cytometry Core facility.

My most important contribution as a technical expert and collaborator has led to the discovery of the TRIM5伪 protein (capable of blocking HIV infection in monkey cells), a groundbreaking advance in AIDS research published by M. Stremlau in the journal Nature in 2004.听听This study has been widely reported in the mainstream press and has been cited over 800 times in scientific journals since publication.听 Since then,听I have acquired a broad knowledge in Immunology, first at the Division of Viral Pathogenesis at Beth Israel Deaconess Medical Center, and at Boston College. First, I worked extensively on measuring immune response on lymphocyte subsets during HIV or SIV infection studies in humans and monkeys. Then at Boston College, I focused my work on monocyte/macrophage subset, a cell population thought to bring HIV or SIV in the brain that induce encephalitis and neuronal damage.

As a Director of the 51动漫 flow cytometry facility, I interact with many researchers and students. In addition, I manage the scheduling of the facility, the budget and the ordering of reagents. Finally, as a collaborator I helped many researchers designing their flow cytometry experiment so that their research project would be successful.

A full bibliography of my publications is available听

Top Cited Research:听

1) Stremlau M, Owens CM, Perron MJ, Kiessling M, Autissier P and Sodroski J. (2004). 淭he cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World Monkeys. Nature, 427 (6977): 848- 53. (Cited by 2445 Google Scholar)听

2) Ancuta P, Kamat A, Kunstman KJ, Kim EY, Autissier P, Wurcel A, Zaman T, Stone D, Mefford M, Morgello S, Singer EJ, Wolinsky SM, Gabuzda D. (2008). 淢icrobial translocation is associated with increased monocyte activation and dementia in AIDS patients. PLoS One, 3(6): e2516. (Cited by 627)听

3) Burdo TH, Lentz MR, Autissier P, Krishnan A, Halpern E, Letendre S, Rosenberg ES, Ellis RJ, Williams KC. (2011). 淪oluble CD163 Made by Monocyte/Macrophages Is a Novel Marker of HIV Activity in Early and Chronic Infection Prior to and After Anti-retroviral Therapy. J. Infect. Dis. Jul;204(1): 154-63. (Cited by 425)听

4) Patrick Autissier, Caroline Soulas, Tricia H. Burdo, Kenneth C. Williams. (2010). 淓valuation of a 12- color flow cytometry panel to study lymphocyte, monocyte, and dendritic cell subsets in humans. Cytometry A, May, 77(5): 410-9. (Cited by 336)

Flow Cytometry
Patrick Autissier, Ph.D
Director, Flow Cytometry Facility
617-552-1417
autissie@bc.edu
Higgins Hall: Room 445A

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